A Rat Model of Rheumatoid Arthritis: TDZD-8 is Associated with the Protection Against the Induction of the Synovium Knee Joint IL-17A/GSK3β/ROS/α-SMA Axis of Fibrosis

Rheumatoid arthritis (RA) that affects the synovial knee joint causes swelling of membrane and tissue damage. Interleukin-17A (IL-17A) enzyme glycogen synthase kinase-3β (GSK3β) are involved in pathogenesis RA. The link between IL-17A, GSK3β, oxidative stress, profibrogenic marker alpha-smooth muscle actin (α-SMA) with without TDZD-8, GSK3β inhibitor has not been studied before. Consequently, active immunization rats was performed to induce RA after three weeks using collagen type II (COII) injections. treated group received daily injection 1 mg/kg TDZD-8 for 21 days following protocol (COII+TDZD-8). Blood synovium samples were harvested at end experiment. development confirmed as corroborated by a substantial increase blood levels highly specific autoantibody RA, anti-citrullinated protein antibody well augmentation reactive species (ROS) measured lipid peroxidation. induction also increased IL-17A marker, α-SMA. All these parameters seemed be significantly (p<0.0001) ameliorated TDZD-8. Additionally, significant correlation ROS, α-SMA biomarkers observed. Thus, augmented IL-17A/GSK3β/ROS/α-SMA axis mediated rat model which inhibited